High Performance Liquid Chromatography | Retention time HPLC
HighPerformanceLiquidChromotagraphy(HPLC)isananalyticaltechniqueusedfortheseparationofcompoundssolubleinaparticularsolvent.HistoryofHPLCLiquidchromatographywasinitiallydiscoveredasananalyticaltechniqueintheearlytwentiethcenturyandwasfirstusedasamethodofseparatingcoloredcompounds.Thisiswherethenamechromatographychromameanscolor,graphymeanswriting,wasderived.ARussianbotanistnamedMikhailS.Tswettusedarudimentaryformofchromatographicseparationtopurifymixturesofplantpigmentsintothepureconstituents...
High Performance Liquid Chromotagraphy (HPLC) is an analytical technique used for the separation of compounds soluble in a particular solvent.
History of HPLCLiquid chromatography was initially discovered as an analytical technique in the early twentieth century and was first used as a method of separating colored compounds. This is where the name chromatography chroma means color, graphy means writing, was derived. A Russian botanist named Mikhail S. Tswett used a rudimentary form of chromatographic separation to purify mixtures of plant pigments into the pure constituents. He separated the pigments based on their interaction with a stationary phase, which is essential to any chromatographic separation. The stationary phase he used was powdered chalk and aluminia, the mobile phase in his separation was the solvent. After the solid stationary phase was packed into a glass column (essentially a long, hollow, glass tube) he poured the mixture of plant pigme...