Expression of small hairpin RNA by lentivirus | shluc
RNAinterference(RNAi)isasequence-specificRNAdegradationprocessmediatedbyshortdouble-strandedRNAstermedsmallinterferingRNAs.Here,wedescribethelentivirus-basedvectorsmallinterferingRNAsystemexpressing.Asapilotstudy,wegeneratedconstructsexpressingsmallhairpinRNA(shRNA)specificforluciferasegene(shLuc)orgreenfluorescenceprotein(shGFP)underthecontrolofhumanH1RNApolymeraseIIIpromoter.TheeffectoftheshRNAwasevaluatedagainstHIV-1infectioninasingle-roundormultiple-roundinfectioussystemusinganHIV-1molec...
RNA interference (RNAi) is a sequence-specific RNA degradation process mediated by short double-stranded RNAs termed small interfering RNAs. Here, we describe the lentivirus-based vector small interfering RNA system expressing. As a pilot study, we generated constructs expressing small hairpin RNA (shRNA) specific for luciferase gene (shLuc) or green fluorescence protein (shGFP) under the control of human H1 RNA polymerase III promoter. The effect of the shRNA was evaluated against HIV-1 infection in a single-round or multiple-round infectious system using an HIV-1 molecular clone carrying the luc or GFP gene. In the single-round infectious system, cells transduced with shLuc by lentiviral vector significantly reduced (approximately 90% reduction) viral gene expression after challenge infection at a multiplicity of infection of 10. These transduced cells continued to resist against at least four sequentially repeated challenge infec...