A New Hybrid System Capable of Efficient Lentiviral Vector ... | lentivirus adenovirus
AbstractToachieveefficientandsustainedgeneexpression,wedevelopedanewlentivirus/adenovirushybridvector(LAvector)thatencodessequencesrequiredforproductionofahumanimmunodeficiencyvirus-basedlentiviralvector(i.e.,alentiviralvector,agag/pol/revexpressioncassette,atetracycline-inducibleenvelopecassette,andthetetracycline-inducibletranscriptionalactivatorcassette)inasinglehelper-dependentadenovirusvectorbackbone.Viaeithertransfectionorinfection,humancelllinestransducedwiththeLAvectorproducedalentiv...
AbstractTo achieve efficient and sustained gene expression, we developed a new lentivirus/adenovirus hybrid vector (LA vector) that encodes sequences required for production of a human immunodeficiency virus-based lentiviral vector (i.e., a lentiviral vector, a gag/pol/rev expression cassette, a tetracycline-inducible envelope cassette, and the tetracycline-inducible transcriptional activator cassette) in a single helper-dependent adenovirus vector backbone. Via either transfection or infection, human cell lines transduced with the LA vector produced a lentiviral vector in a doxycycline-dependent manner at titers up to 105 to 106 green fluorescent protein transducing units per ml, which are comparable to the titers obtained by conventional multiple plasmid transfection methods. Efficient spread and persistent expression of the transgene were observed in cells maintained in long-term culture that had been infected with the LA vector. Furthermore, when...